Fig. 3.

TIDCs cannot present tumor-derived antigen in vitro. We injected mice with B16.OVA or control B16.F10, excised the tumors after 14 days (size of tumors 0.5–1.5 cm²) and digested them with collagenase. Cell suspensions were pre-enriched for CD45+ cells by magnetic bead sorting and electronically sorted for CD11c⁺ DC. a The sorting strategy and purity of TIDCs are shown. TIDCs contain melanin that can be seen as black particles inside the sorted DCs. b Graded doses of tumor-derived DC were cocultured with 2 × 10⁵ OVA-specific CD4+ OT-II and CD8+ OT-I T cells for 3 days, and radioactive thymidine was added during the last 16 h. Results are expressed as counts per minute (cpm). Cultures were set up in triplicate; mean ± SD are shown. Results are from one of four experiments that gave similar results