Fig. 2.

Cytotoxicity of human PBL by ds-Diabody or parent diabody in a non-radioactive cytotoxicity assay. Cytotoxicity of PBL towards K562/A02 cells mediated by ds-Diabody in different concentrations at various E:T ratios (a). Cytotoxicity of PBL towards K562/A02 cells (b) or K562 cells (c) at different E:T ratios in the presence of PBS, a mixture of the PHMA02 scFv and HIT3a scFv, ds-Diabody, or the parent diabody. The antibody concentration used was 400 ng/mL. Data shown are the mean ± SD of triplicate determinations