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Flow cytometric analysis of reactivity of bsAb HD37xT5.16. Binding of indicated monoclonal antibody and bispecific antibody to Jurkat (CD5+ and CD19−) and Raji (CD5− and CD19+) cells was detected with isotype-specific, FITC-labeled second-step reagents. The gray histogram represents a negative control with irrelevant monoclonal antibody HD20
