Fig. 7.

Cytotoxic activity of NK cells against primary chronic lymphocytic leukemia cells. CLL cells were obtained from routine peripheral blood samples of untreated patients. Mononuclear cells were enriched by density gradient centrifugation, analyzed for expression of CD20 by flow cytometry, and directly used for cytotoxicity experiments. Cytotoxic activity of CD20-specific NK-92-scFv(Leu-16)-ζ and parental NK-92 cells was analyzed as described in the legend of Fig. 2. Mean values of triplicate samples are shown. The standard deviation is indicated by error bars