Fig. 1.

Efficiency of DC infection and CD8/CD25 depletion. a B16 cell line expressing h5T4 is stained for h5T4 as a positive control and h5T4 expression on dendritic cells (73.4%) infected with Ad-h5T4. CD4⁺-enriched subpopulations were assessed by FACS analysis for contamination with residual CD8⁺ T cells (<0.4%, b) or CD25+ cells (<0.1%, c)