Fig. 2.

Depletion of CD25⁺ cells prior to cell expansion significantly enhances h5T4-specific CD4⁺ T-cell responses in healthy individuals. CD4⁺-enriched PBLs undepleted or depleted of CD25⁺ cells were expanded in vitro for two stimulations using h5T4-expressing DC. The reactivity of expanded effectors against autologous PBMC pulsed with h5T4-derived peptide pools is measured in IFN-γ ELISPOT assays. The data are means of triplicate determinations ±SE. NC is negative control where expanded effectors were incubated with media alone