Fig. 5.

Response against P3 is generated in HLA-DR4 positive healthy donors following polyclonal stimulation. CD25⁺-depleted CD4⁺-enriched PBLs were expanded twice with h5T4-expressing DC. They were then incubated with DMSO (to calculate background), P1, P2, or P3 15-mer peptides for 6 h. IFN-γ secretion from CD3⁺CD4⁺ T cells was assessed by flow cytometric analysis. 0.25% of CD3⁺CD4⁺ T cells secreted IFN-γ in response to P3 but not to P1 or P2 peptides. One representative experiment of two performed is shown