Table 2.
Summary of h5T4-specific CD4+ T-cell responses in nine healthy donors
| Peptide | Pool 1 | Pool 2 | Pool 3 | Pool 4 | Pool 5 | Pool 6 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor | CD25+ | CD25− | CD25+ | CD25− | CD25+ | CD25− | CD25+ | CD25− | CD25+ | CD25− | CD25+ | CD25− |
| HD1 | 7 | 7 | 9 | 6 | 5 | 8 | 12 | 202 | 19 | 2 | 5 | 5 |
| HD2 | 0 | 8 | 0 | 5 | 6 | 12 | 0 | 32 | 0 | 6 | 0 | 13 |
| HD3 | 1 | 0 | 40 | 213 | 25 | 51 | 21 | 63 | 10 | 6 | 9 | 0 |
| HD4 | 1 | 21 | 0 | 9 | 0 | 16 | 0 | 17 | 0 | 11 | 0 | 1 |
| HD5 | 0 | 2 | 0 | 4 | 2 | 4 | 1 | 14 | 1 | 4 | 0 | 4 |
| HD6 | 8 | 4 | 0 | 3 | 0 | 13 | 0 | 4 | 0 | 3 | 3 | 28 |
| HD7 | 0 | 0 | 0 | 0 | 49 | 78 | 0 | 0 | 0 | 0 | 31 | 54 |
| HD8 | 0 | 0 | 0 | 0 | 3 | 12 | 0 | 2 | 0 | 0 | 0 | 21 |
| HD9 | 2 | 3 | 0 | 0 | 5 | 2 | 3 | 4 | 0 | 0 | 7 | 0 |
h5T4 specific IFN-γ-producing CD4+ T-cell responses to 6 pools of h5T4 32-mer peptides following 2 rounds of stimulation with autologous DC infected with Ad-h5T4. Specific h5T4-peptide responses were calculated by subtracting the mean number of spots in CD4+ T cells plus PBMC (in the absence of peptides). Specific frequencies are shown per 105 cells. Bold indicates donors where a frequency >1/104 and with an increase in the CD25+-depleted CD4+ T responders compared with undepleted cells
HD Healthy donor, CD25 + undepleted population, CD25 − CD25-depleted population