Table 1.

Enrichment and amplification yields in Melan-A, GP100 and NA17-A specific T cells after MHC/peptide multimer sorting

	Melan-A								GP-100	NA17-A
	Pt 1 #1 (PBMC)		Pt 1 #2 (PBMC)		Pt 2 (PBMC)		Pt 3 (TIL)		Pt 5 (PBMC)	Pt 6 (PBMC)
Starting population
Total cell number (×106)	4		4		5		4		10	2.8
CD8 specific cells (%)a	2		44		0.24		6.8		0.23	0.1
CD8 specific cells nbb	8 × 104		1.76 × 106		1.2 × 103		2.72 × 105		2.3 × 104	2.8 × 103
D14 after sort/amplification
Cell sorting condition	M450-AvT	M280s	M450-AvT	M280s	M450-AvT	M280s	M450-AvT	M280s	M450-AvT	M450-AvT
Amplified cell nb (×106)	380	210	751	1,000	15.4	23.4	150	120	26	1.2
CD8 specific cells (%)a	96	88	99	96	95	89	98	96	90	95
CD8 specific cells nbb	3.65 × 108	1.85 × 108	7.43 × 108	1.1 × 109	1.42 × 107	1.78 × 107	1.47 × 108	1.15 × 108	2.34 × 107	1.08 × 106
Enrichment factorc	4,563	2,313	422	625	1,183	1,483	529	415	1,017	386

^aPercentage of T cells specific for HLA*A201/peptide complexes is determined by CD8/tetramer double labeling

^bNumber of specific cells (percentage of tetramer labeled cells multiplied by the total number of cells)

^cEnrichment factor is the ratio between the number of specific cells after and before one sort/amplification cycle