Table 6.
Blocking of CTL020 cell migration in reconstruct
| Treatmenta | Total number of tumor cells mean ± SD/field (10 fields) | Number of apoptotic tumor cells mean ± SD/field (10 fields) | Percentage of apoptotic tumor cells mean ± SD/field (10 fields) | Percentage of tumor cell apoptosis inhibition |
|---|---|---|---|---|
| WC020 | 69.9 ± 15.8 | 11.1 ± 3.4 | 15.8 ± 2.5 | NA |
| WC020 + CTL020 | 58.9 ± 7.5 | 23.9 ± 4.2 | 40.8 ± 6.9b | 0.0 |
| WC020 + CTL020 + control IgG | 49.3 ± 9.1 | 18.5 ± 4.0 | 38.9 ± 6.9c,d | 4.7 |
| WC020 + CTL020 + α-CXCR3 Ab | 72.7 ± 7.0 | 18.0 ± 4.3 | 24.9 ± 6.2c | 39.0 |
| WC020 + CTL020 + α-CCR7 Ab | 59.7 ± 8.2 | 24.0 ± 5.2 | 39.8 ± 4.6 | 2.5 |
| WC020 + CTL020 + α-CCR9 Ab | 59.9 ± 11.1 | 21.7 ± 4.8 | 36.4 ± 6 | 10.8 |
| WC020 + CTL020 + α-CXCR4 Ab | 60.4 ± 5.1 | 23.1 ± 5.2 | 38.7 ± 8.7 | 5.2 |
| WC020 + CTL020 + CXCL11 | 69.4 ± 6.8 | 20.1 ± 2.5 | 29.1 ± 4.8b | 28.7 |
| WC020 + CTL020 + α-CXCL11 Ab | 74.8 ± 6.0 | 17.4 ± 5.7 | 23.4 ± 8.3d | 42.7 |
Ab antibody, NA not applicable
aReconstructs consisted of a bottom layer of collagen and fibroblasts, followed by a tumor cell layer and a separating layer of collagen and fibroblasts. Anti-chemokine or anti-chemokine receptor or control Abs were added at 10 μg/ml, followed by a top layer containing CTL mixed with fibroblasts and collagen (E:T = 3:1). Separate cultures received CXCL11 or CXCL9 (50 ng/ml) on top of the T-cell layer. Percentage of apoptotic tumor cells in 6-day cultures was determined
b–dValues with same letter differ significantly from each other (P < 0.01, Student’s two-sample t-test)