Fig. 3.

Tumor infiltrating T-lymphocytes were found in mice that received DOTAP/E7 at an optimal dose. TC-1 tumors were established as described and were left untreated or given treatment on day 6. Solid tumors were dissected on day 14 and examined for infiltrating lymphocytes. FITC conjugated anti-CD8 (a, b, c) and anti-CD4 (d, e, f) antibodies were used to determine tumor infiltrating T cells followed by counterstaining with DAPI. Representative tumor sections from groups of three mice were examined as described and imaged by confocal microscopy. TUNEL assay was conducted to detect cell apoptosis in the tumor sections (g, h, i)