Fig. 6.
High dose of cationic DOTAP liposome induces ROS production, resulting in cell death in DC. a DLNs from mice (n = 4) that received injection sc of DOTAP/E7 containing 0, 15, 100 or 600 nmol lipid were isolated 2 h after the injection. The total lymph node cells were incubated with DCFH-DA at 37°C for 30 min prior to the flow cytometric analysis. Large granular cells were gated and analyzed for the expression of ROS, where the fluorescent product DCF was generated in the presence of ROS. The relative percentages of cells with positive ROS signals are listed. b Cytotoxicity in the DLN was measured by collecting cells at 10 h after DOTAP/E7 injection by flow cytometry. The relative percentages of dead cells (PI positive) in DC (open square) and the numbers of live DC per 105 LN cells (bars) were shown and compared with that of the untreated control by paired Student’s t test (**P < 0.01)