Fig. 10.

Effect of decoy ODN on cytokine-mediated MHC class I and II antigen expressions. Double-stranded, decoy ODN were prepared from complementary single-stranded, phosphorothioate-bonded bases. Decoy ODN were added 1 day after seeding) to the wells for 24 h. IFN-γ (1,000U/ml) and IL-6 (10 ng/ml) were added to the CTVT cells pretreated with decoy ODN. After incubation at 37°C for 72 h, MHC class I and II antigen expressions were d ODN (CRE, STAT-1, STAT-3, IRF-1, NF-κB) significantly decreased the expression of MHC class I and II molecules on the surface of tumor cells exposed to IFNγ/IL-6 for 72 h. Filled profile: isotype control. Open profile: with ODN (bold line) and without ODN (light line). Data are representative of three experiments