Fig. 1.
a Linearity of the P815-dependent generation of IFN-γ and IL-4-producing and spot-forming cells as detected by our ELISPOT assay. Spleen cells from P815-bearing mice were plated at various dilutions, together with spleen cells from normal mice, to keep the total number of spleen cells constant at 2×106 cells per well with or without 5×105 γ-irradiated (20,000 rads) P815 cells as antigen. Antigen-dependent IFN-γ and IL-4-producing cells were enumerated. b Linearity of the L5178Y-dependent generation of IFN-γ and IL-4 producing, spot-forming cells as detected by our ELISPOT assay. Spleen cells from L5178Y-primed mice were plated at various dilutions, together with spleen cells from normal mice to keep the total number of spleen cells constant at 1×106 cells per well. As antigen, 105 γ-irradiated (5,000 rads) L5178Y cells were added to appropriate wells. Antigen-dependent IFN-γ and IL-4 producing cells were enumerated