Fig. 1.

Assessment of LC-induced killing of canine MH cells in vitro. Quadruplicate wells containing 4 × 10³ MH cells per well were treated with the indicated concentration of liposomal clodronate (LC) or PBS liposomes (L-PBS), incubated for 72 h, then cell viability was assessed by MTT assay, as described in “Methods”. Results are reported as the mean (±SEM) percentage killing. In a–c, the dose-responsiveness of DH82 cells, MH-1 cells, and MH-2 cells, respectively, to incubation with LC and with L-PBS is depicted. Differences in the percentage of killing elicited by treatment with LC and L-PBS were compared at each time point using Student’s t test. (* denotes statistically significant differences of p < 0.05). These data are representative of three independent experiments