Fig. 3.

Susceptibility of phagocytic and non-phagocytic tumors to killing by LC. Killing of canine MH cell lines (DH82, MH-1, MH-2) by LC was compared to killing of three non-phagocytic canine tumor cell lines (thyroid carcinoma {CTAC}, malignant melanoma {Mel-J} and osteosarcoma {D17}). The percentage specific killing was determined as the difference between the percentage killing elicited by LC and the percentage killing elicited by PBS liposomes for all cell lines shown. In (a), dose response curves of LC percentage specific killing were determined for the DH82 cell line and three non-phagocytic tumor cell lines (CTAC, D17, Mel-J). In (b), the percentage specific killing elicited by 5% LC was plotted for MH cells (DH82) and three non-MH tumor cell lines. Specific killing was significantly greater (*, p < 0.05) for DH82 cells than for the other three tumor cell lines, as assessed by ANOVA, followed by Bonferroni’s post-test. In (c), LC-specific killing of three different MH cell lines was compared, using LC at a concentration of 5%. Killing was significantly greater (*, p < 0.05) for DH82 cells than for the other two MH cell lines, as assessed by ANOVA with Bonferroni’s post-test. The data shown are representative of three independent experiments