Fig. 6.

Effect of R-848 on killing of primary AML cells by allogeneic PBMC or NK cells. Flow cytometric detection of AML cell viability following coculture of primary AML cells and allogeneic PBMC (black bars) or NK cells (striped bars) in the presence of R-848. PKH67-positive cells that were positive for annexin V and/or PI were defined as non-viable AML cells. (a) Coculture of effector cells with primary AML cells from AML P17. (b) Coculture of effector cells with primary AML cells from AML P16. Results are representative of experiments done with two different effector cell donors.+ R-848, addition of R-848; + Ab, addition of neutralizing antibodies directed against IL-6 and TNF-α; + R-848 + Ab, addition of both R-848 and neutralizing antibodies against IL-6 and TNF-α