Fig. 2.

a IDO expression in correlation to the maturing process with IL-1β, TNF-α, IL-6 and PGE-2 of human DCs (representative example). Expression of IDO mRNA relative to the housekeeping-gene GAPDH is depicted. Filled square Mature DCs, open square Immature DCs. b Western blot analysis of protein lysates of immature (−) and mature (+) human dendritic cells. c Parallel measurement of tryptophan concentration in supernatant, obtained at the given time points after the induction of maturation (filled bars) or not (open bars). Similar results were obtained on repetitive experiments