Fig. 1.

a, b Cytokine and COX-2 detection on different SCC and AD cell lines. a AD cell lines (A-549, A-427, SK-LU-1, and 3B1A) and SCC cell lines (SK-MES-1 and Calu-1) were cultured at 10⁶  cells/10 ml for 24 h. Then, total RNA was extracted and RT-PCR for the indicated molecules and β-actin was performed. Agarose gel electrophoresis of RT-PCR fragments is shown from a representative experiment. b Analysis of IL-6 and TGF-β1 production by A-427 (AD) and Calu-1 (SCC) cell lines. Cells were harvested after 24 h of culture, and cytokines in the supernatants were evaluated by ELISA. Results are the mean ± SD of three independent experiments. Statistical analysis (AD versus SCC): ^* p<0.05