Fig. 7.

a, b Effect of IL-6, IL-10, TGF-β1, and tumor-derived prostanoids on DC ability to promote cytokine secretion on allogeneic naïve CD4⁺ T lymphocytes. DC were obtained as in Fig. 6, and co-cultures with T lymphocytes were established as described in Materials and methods. Cytokines in culture supernatants were determined by ELISA. a Analysis of IFN-γ secretion on culture supernatants of lymphocytes stimulated with Ctrl-DC (white bars) or A-427-DC (black bars). b Analysis of IL-10 on culture supernatants of lymphocytes stimulated with Ctrl-DC (white bars), A-427-DC (black bars) or Calu-1-DC (gray bars). DC treatments are indicated on the x-axis in a and b, and results are the mean ± SD of three independent experiments. Statistical analysis (tumor cell-treated DC versus Ctrl-DC): ^* p<0.05