Fig. 5.

T lymphocytes from protected mice secrete IFNγ after stimulation with bmDCs incubated with B16 lysates. T cells derived from spleens taken from surviving mice 8 weeks after B16 inoculation or from normal mice were purified using MACS beads. These cells were further stimulated with bmDCs previously incubated with: B16 cell lysates, GM3 ganglioside or empty bmDCs, as described in “Materials and methods”. After 96 h culture, supernatants were harvested and IFNγ release was measured by ELISA. No IFNγ was produced by T cells from normal mice. The mean ± SD of IFNγ determinations from five mice in the vaccinated tumour-free animals and three mice in the reference group are shown. Statistical analysis was performed using the Mann–Whitney test (p < 0.05). One representative experiment of three is shown