Fig. 8.

The effect of treating ovarian cancer cell lines with 10 μM DAC for 7 days on recognition by antigen-specific CD8⁺ T lymphocytes. The indicated ovarian cancer cell lines were incubated for 7 days in the presence or absence of 10 μM DAC and were then harvested. The cells were then used as stimulator cells in an interferon-γ ELISpot assay. CD8⁺ T lymphocytes reactive to the NY-ESO-1-dervied peptide ASG presented in association with HLA-A3 (a–d), for the MAGE-A1-derived peptide SLF presented in association with HLA-A3 (e–h), and the MAGE-A10-derived peptide GLY presented in association with HLA-A2 (i, j) were used. C1R-A2 and C1R-A3 cells pulsed with the relevant peptide served as a positive control, while C1R-A2 and C1R-A3, either unpulsed or pulsed with a control peptide (GAG), served as negative controls. Each panel of ovarian cancer cells used as stimulators included three lines that were HLA-matched for the relevant class I MHC molecule and one line that was HLA-unmatched for the relevant class I MHC molecule. The data are presented as the mean ± SEM for two independent experiments