Table 1.

The effect of treating ovarian cancer cell lines with DAC on CTA gene expression

DAC treatment	No. of cell lines with an increase/No. of cell lines where an increase could be measured
Conditions	MAGE-A1	MAGE-A2	MAGE-A3	MAGE-A4	MAGE-A6	MAGE-A10	MAGE-A12	NY-ESO-1	TAG-1	TAG-2a	TAG-2b	TAG-2C
1 μM, 3 daya	8/11	0/11	1/9	2/11	0/11	1/10	0/9	9/11	2/10	3/11	2/11	3/11
10 μM, 7 dayb	9/9	0/11	9/9	11/11	7/11	9/9	8/9	10/10	8/9	5/9	8/11	4/11

DAC treatment	No. of CTA with an increase/No. of CTA where an increase could be measured
Conditions	CAOV-3	CAOV-4	COV413	ES-2	OV-90	OVCAR-3	SK-OV-3	SW626	TOV-21G	TOV-112D	TTB-6
1 μM, 3 daya	2/12	3/12	7/12	5/10	0/10	3/10	1/12	2/12	2/12	5/12	1/12
10 μM, 7 dayb	9/12	11/12	10/12	7/9	1/7	6/9	10/12	9/12	10/12	9/10	6/10

Eleven ovarian cancer cell lines were incubated in the presence or absence of DAC under the indicated treatment conditions. The expression levels of the 12 indicated CTA genes were determined by PCR as indicated in the “Materials and methods”

^aTwo independent experiments were performed for this treatment condition. An increase in expression following DAC treatment was defined as an increase of two or more units on the relative expression scale in each of the two experiments. When a particular CTA/cell line pair could not show an increase because the untreated cells in at least one experiment had a relative expression level of 7 or 8 on a scale of 8, these data points were excluded from the denominator

^bThree independent experiments were performed for this treatment condition. An increase in expression following DAC treatment was defined as an increase in expression in each individual experiment and an increase of two or more units on the relative expression scale in at least two of the experiments. When a particular CTA/cell line pair could not show an increase because the untreated cells in at least one experiment had a relative expression level of 7 or 8 on a scale of 8, these data points were excluded from the denominator