Fig. 1.

Flow cytometric analyses of PBMCs by FACS can. The figures are representative of a pancreatic cancer patient. Region R1 includes lymphocytes and monocytes but excludes debris. DCs are detected in region R2 as the population of Lin⁻/HLA-DR⁺ and divided into two fractions by the expression of CD11c (region R3; CD11c⁺ DC (DC1) and region R4; CD11c⁻ DC (DC2)). The NK cell fraction is gated in the CD14⁻/CD56⁺ population (region R5). CD3⁺/CD4⁺ T lymphocytes are detected in region R6 and CD3⁺/CD8⁺ T lymphocytes are detected in region R7