Fig. 1.

T cell clone selection and characterization. a TNF secretion by M170.51 T cell clone in response to the autologous melanoma cell line. 10⁴ CTL were added to 3 × 10⁴ M170 melanoma cells, in the presence of blocking antibodies directed against class I, A2 and B/C HLA, diluted to 1/5,000 (thin hatched bars) 1/500 (thick hatched bars) and 1/50 (black bars). b TNF response of M170.51 CTL clone to HLA-A*0201 tumor cell lines. M6 cell line, HLA-A2 negative, was used as a negative control. 09M08 and 01M08 were HLA-A*0201 melanocyte cell lines