Table 1.

Sequences of primers used ORFs subcloning

Primers	5′–3′ sequencesa
ORF subcloning
(285–404) forward	aaaGAATTCgccaccATGAGTGAAAATGCAGGAGG
(285–404) reverse	aaaCTCGAGTCACTGGCACAGTGCAG
(486–689) forward	aaaGAATTCgccaccATGTCCGTAGGGAGACGC
(486–689) reverse	aaaCTCGAGTCACCCTAGAGCTGCCAAG
(1105–1194) forward	aaaGAATTCgccaccATGAAATTTGAATTAATTTGCAGAAC
(1105–1194) reverse	aaaCTCGAGTTAATTCCTAAGGCATTCATTC
(1230–1370) forward	aaaGAATTCgccaccATGAGTTGTGTAGGTTATCC
(1230–1370) reverse	aaaCTCGAGTCACAGGGATGCCGGCC
ORF285–404 partial sequence subcloning
(285–341) forward	AATTCgccaccATGAGTGAAAATGCAGGAGGTGCCGTAGCGAGAACAGCGACAGCATTCTGCGCATTGTGAC
(285–341) reverse	TCGAGTCACAATGCGCAGAATGCTGTCGCTGTTCTCGCTACGGCACCTCCTGCATTTTCACTCATggtggcG
(285–371) forward	AATTCgccaccATGAGTGAAAATGCAGGAGGTGCCGTAGCGAGAACAGCGACAGCATTCTGCGCATTGGTGAGCCCGACTCCCCAGCCTCGGTGCCCATGAC
(285–371) reverse	TCGAGTCATGGGCACCGAGGCTGGGGAGTCGGGCTCACCAATGCGCAGAATGCTGTCGCTGTTCTCGCTACGGCACCTCCTGCATTTTCACTCATggtggcG
(345–404) forward	AATTCgccaccATGAGCCCGACTCCCCAGCCTCGGTGCCCACCGAAGCCCCCTCTGGCTGCACTGTGCCAGTGAC
(345–404) reverse	TCGAGTCACTGGCACAGTGCAGCCAGAGGGGGCTTCGGTGGGCACCGAGGCTGGGGAGTCGGGCTCATggtggcG

^a Italics enzyme restriction sites, lower case Kozak sequence