Fig. 1.

Autophagy negatively regulates MHC-I antigen expression. a, b Autophagy decreases MHC-I expression in macrophage. The cultured peritoneal macrophages were treated with rapamycin (0.5 μg/ml), 3-MA (3 mM), BM (10 nM), wortmannin (100 nM), or chloroquine (25 μM) for 24 h. The MHC-I antigen positive cell proportion (a) and the mean fluorescent intensity MFI (b) were analyzed by flow cytometry. c Atg5, Atg7, and becalin 1 siRNAs were transfected into macrophages, respectively. 24 h later, the MFI of MHC-I antigen were analyzed by flow cytometry. The data in this figure are the representatives of three independent experiments. *P < 0.05, compared with control group