Fig. 2.

AFP specific cytotoxic T cell response is induced by the AFP/HSP70 vaccine. a AFP positive EL4 target cells were transfected with pcDNA3.1-AFP, and selected with G418 (right panel). AFP expression on EL4 cells was confirmed by immunohistochemical staining (left panel). b A T cell-specific cytotoxity assay against AFP-positive EL4 target cell was performed. Splenocytes, enriched for CD8+ cells were isolated from vaccinated mice 14 days after immunization. A LDH release assay was performed at different effector-to-target cell ratios with splenocytes being re-stimulated with either inactivated EL4 or AFP⁺EL4 cells for 1 week, while EL4 or AFP⁺EL4 cells served as target cells