Fig. 4.

Identification of the LAGE-1 antigenic peptide. The LAGE-1b cDNA is shown in the upper part of the figure with exons appearing as open boxes, the spliced intron as kink, and the open reading frame as black boxes. Truncated LAGE-1b cDNA were co-transfected into COS-7 cells together with an HLA-A*68012 cDNA. The transfected cells were tested for recognition by the CTL. Several peptides encoded by this region were tested for recognition by CTL E8: HLA-A68⁺ chromium-labeled EBV-B cells were incubated for 15 min with each peptide at different concentrations, before addition of clone E8 at an E:T ratio of 5:1. Chromium release was measured after 4 h. The concentrations indicated in the figure are the concentrations during the 4 h of incubation