Fig. 5.

Lysis of tumor cell lines by CTL clone E8. a Cell line LB1965-EBV.retroLAGE-1b has been obtained by transduction of EBV-B cells with a retrovirus carrying a complete LAGE-1 coding sequence. LB831-BLC is a bladder carcinoma cell line. The A68-transfected cell line is a clonal population of cells stably transfected with an HLA-A*68012 cDNA. Target cells were ⁵¹Cr-labeled for 1 h, if indicated, pulsed for 15 min with 5 μM of peptide ELVRRILSR and incubated with CTL at various E:T ratios. The chromium release was measured after 4 h. b Unlabeled competitors were added before adding CTL at an E:T ratio of 5