Fig. 5.

Proliferation of immunized spleen cells in response to antigens in vitro. T-cell proliferation was determined by [³H]thymidine incorporation, and proliferation in the presence of media alone has been deducted from values obtained in the presence of different stimulants. a, b Splenocytes were obtained from groups of mice as indicated and cells were cultured in the presence of different stimulants. c Analysis of subsets of T cells in splenocytes obtained from mice immunized with 3H1-CpG or CEA-CpG. 3H1–CpG-immunized mice splenocytes were cultured in the presence of 3H1 and CEA–CpG-immunized mice splenocytes were cultured in the presence of CEA. Antibodies (10 μg/ml) against the indicated antigens were added at the beginning of culture. Results in a–c are the mean ± SE of one representative experiment of three performed experiments