Table 2.

Th1-associated immune response in splenocytes from immunized mice^a

Immunizations with	mIL-2	mIFN-γ	mIL-4	mIL-10
3H1-QS21	112.0±16	430.0±20	10.0±2	16.0±8
CEA-QS21	64.0±8	180.0±10	10.0±2	8.0±8
3H1-CpG	336.0±24*	925.0±30**	36.0±6	56.0±16
CEA-CpG	208.0±16	410.0±15	24.0±4	40.0±8
1A7-CpG	40.0±8	145.0±10	16.0±2	24.0±8
PBS	16.0±8	50.0±5	6.0±2	8.0±8

Results are expressed in pg/ml

^aSplenocytes (2×10⁵ /well) obtained from groups of immunized mice were cocultured in the presence of 1.0 μg of 3H1 or CEA in 96-well flat-bottomed microtiter plates in a final volume of 200 μl. Cell-free supernatants were harvested after 48 h (for quantitation of IL-2, IL-4, and IL-10) and 72 h (for quantitation of IFN-γ) of culture and cytokine levels were measured by standard ELISA kits. One representative experiment of two is shown

^* P < 0.001 with respect to other experimental groups for IL-2 production

^** P < 0.005 with respect to other experimental groups for IFN-γ production