Fig. 4.

Effects of Gö6983 on the expression of death ligands and cytotoxic molecules in primary NK cells. a Expression of death ligands on primary NK cells were determined by RT-PCR and flow cytometry analysis. b Expression levels of perforin and granzyme B mRNAs and proteins in primary NK cells after 5 μM Gö6983 treatment for 4 h were determined by RT-PCR and intracellular flow cytometry. Dotted line isotype control, Black line none, Filled Gö6983 treatment)