Table 5.
Intracytoplasmic cytokine synthesis by cancer antigen peptide-specific T cell lines generated from PBMC and stimulated with the relevant GCAA peptide
| Patients | Proportion (%) of peptide-reactive T cell blasts expressing cytokines | |||
|---|---|---|---|---|
| CD4+ IFN-γ+ | CD4+IL-4+ | CD8+ IFN-γ+ | CD8+IL-4+ | |
| 02.CC | 11.2 | 0.11 | 15.1 | 0.19 |
| 03.CA | 12.1 | <0.1 | 12.8 | <0.1 |
| 04.FM | 6.2 | <0.1 | 10.3 | <0.1 |
| 05.PF | 10.0 | 0.10 | 11.9 | 0.18 |
| 06.CAn | 11.9 | <0.1 | 16.3 | 0.20 |
| 08.DN | 10.9 | 0.20 | 13.9 | 0.10 |
| 09.BG | 7.8 | <0.1 | 10.0 | <0.1 |
| 10.BA | 10.3 | <0.1 | 13.0 | <0.1 |
| 11.BS | 8.1 | <0.1 | 9.1 | <0.1 |
| 12.BG | 8.9 | <0.1 | 10.8 | <0.1 |
| 13.MS | 11.9 | 0.10 | 12.7 | 0.17 |
| 14.AC | 7.0 | <0.1 | 10.1 | <0.1 |
| 17.MM | 7.2 | <0.1 | 10.3 | 0.39 |
| 18.SB | 11.8 | 0.19 | 15.0 | <0.1 |
| 19.AB | 15.1 | 0.18 | 16.2 | <0.1 |
| 20.BF | 10.1 | <0.1 | 11.4 | <0.1 |
Intracytoplasmic IL-4 and IFN-γ positive T cell blasts of PBMC-derived peptide-specific T cell lines. GCAA peptide specific T cell lines were obtained from 16 of 20 patients with gastric adenocarcinoma. T cell blasts were stimulated with peptides (10 μg/ml) and intracellular cytokine synthesis was evaluated by cytofluorometric analysis. Results represent mean proportions of cytokine-positive cells over the background values obtained with isotype-control monoclonal antibodies