Fig. 1.

a Monocytes isolated from peripheral blood mononuclear cells of healthy donors were tested in a transwell assay for their capacity to migrate towards conditioned medium from PC3 or LNCaP cell lines. b Proliferation of macrophages exposed to conditioned media derived from the PC3 and LNCaP prostate-cancer cell lines. Macrophages were propagated from peripheral blood mononuclear cells and exposed to conditioned medium from PC3 or LNCaP cells for 48 h. Their proliferation was evaluated by measuring ³H-thymidine incorporation in the DNA during the last 18 h of incubation. Asterisks denote significant difference over controls (*P < 0.05; **P < 0.01, Students’ t test). Bars represent mean ± SD. Data from three experiments is shown