Fig. 2.

Immunization with hsp110 + CA9 had an antitumor effect. a 2 × 10⁵ RENCA-CA9 tumor cells were injected intradermally 7 days after three immunizations administered 14 days apart (see Materials and methods for dosing information). Each line represents the mean tumor growth in five mice. P values based on repeating measure ANOVA, which takes into account the variability within each group, are provided comparing each group to the control mice injected with PBS. b Immunization with hsp110 + CA9 prevented tumor formation in mice with extended followup. After a total of 40 days, none of the mice immunized with has110 + CA9 developed palpable tumor. c Immunization with hsp110 + CA9 elicited CA9-specific immune responses measured using ELISPOT assay. d Immunization with hsp110 + CA9 elicited CA9-specific humoral response measured using ELISA