Fig. 4.

Immunization with plasmid vector (pcDNA 3.1) containing grp170 linked to CA9 did not prevent tumor growth. a 2 × 10⁵ RENCA-CA9 tumor cells were injected intradermally 7 days after three immunizations (five mice per group) administered 14 days apart. Each line represents the mean tumor growth in five mice. P values are provided comparing each group to the control mice injected with PBS. b Immunization with plasmid vector (pcDNA 3.1) containing grp170 linked to CA9 did not elicit CA9-specific immune responses measured using ELISPOT assay. c Immunization with plasmid vector (pcDNA 3.1) containing grp170 linked to CA9 did not elicit CA9-specific humoral response measured using ELISA