Fig. 1.

Increase in the ex vivo PBMC response to LPS stimulation after vaccination with autologous, antigen-loaded, mature CD83⁺ moDCs. PBMCs harvested before the first (prevaccination) and after the third vaccination (postvaccination) were either left unstimulated (control) or stimulated with the vaccine control antigen KLH (2 μg/ml) or with LPS from S. abortus equi. Proliferative responses (a) were assessed as [³H] thymidine incorporation and cytokines (b and c) were determined in culture supernatants using a Th1/Th2 cytokine bead array (CBA) and flow cytometry. Results are mean values of triplicate measurements ± SD (*P < 0.05–0.01; **P < 0.01–0.001; ***P < 0.001)