Fig. 4.

Enhancement of the ex vivo PBMC response to KLH by LPS. PBMCs were harvested before the first (prevaccination) and after the third vaccination (postvaccination) with autologous, antigen-loaded, mature CD83⁺ moDCs. PBMCs were either left unstimulated (control) or stimulated with LPS (S. abortus equi), the vaccine control antigen KLH (2 μg/ml) or with combinations of KLH and LPS. Proliferative responses were assessed as [³H] thymidine incorporation. Cytokine contents in culture supernatants were determined using a Th1/Th2 cytokine bead array (CBA) and flow cytometry. Results are mean values of triplicate measurements ± SD. One patient representative of three