Fig. 5.

Localized osteomyelitis induces monocytes in the blood and spleen. Mice (n = 5 per group) were challenged with S. aureus osteomyelitis and tumors as previously described. a Mice were tail bled on days 2, 9, and 17 post-tumor challenge. Samples were processed and analyzed by multi-color flow cytometry as described in the Materials and methods and means (±SEM) calculated for each group. There were significant (***p < 0.001, **p < 0.01, *p < 0.05) increases in total blood monocytes at the bracketed measures by two-way ANOVA with Bonferroni post-test. b Analyses from the day two bleed are further characterized showing a significant increase (***p < 0.01) in Ly6C^hi monocytes in infected mice with no difference (p > 0.05) in Ly6C^lo monocytes between groups. c Mice were euthanized on day 21 and spleens were analyzed by flow cytometry as described in the Materials and methods. There was a significant increase (*p < 0.05) in the number of Ly6C^hi monocytes present in the spleen measured by one-way ANOVA with Bonferroni post-test. Results are representative of three independent experiments