Fig. 6.

TCR-RNA-transfected T cells have a similar cytolytic capacity as retrovirally transduced T cells, which approximates cytolytic efficiency of the parental CTL clone. CD8⁺ T cells were electroporated with EGFP RNA (Neg) or RNA coding for the TCR α and β chain and were used as effector cells in cytotoxicity assays 24 h after electroporation. Irradiated T2 cells loaded with different concentrations of gp100_280–288 peptide (as indicated) were used as target cells and percent lysis was calculated. The target to effector cell ratio was 1:15. The peptide concentration corresponding to 50% of the maximum lysis (ED₅₀), used to measure the cytolytic efficiency, is indicated by the dotted line. Average values of triplicates ± SD are shown. Data of one (out of five) representative T cell donor is shown