Fig. 2.

PEP005 stimulated EC demonstrate increased mRNA and protein expression for ICAM-1, E-selectin and IL-8. EC monolayers stimulated with TNF-α or PEP005 were assayed for; a mRNA for ICAM-1 or E-selectin by TaqMan PCR. Both reagents significantly increased the expression of mRNA compared to unstimulated control monolayers. Data are mean ± SEM of five experiments; **P < 0.01 by t test for comparison of TNF-α or PEP005 treated cells with unstimulated cells. b Protein expression of ICAM-1 and E-selectin by ELISA. Data are mean ± SEM of eight experiments. ANOVA demonstrated a significant effect of the concentration of PEP005 on ICAM-1 (P < 0.03) and E-selectin (P < 0.01). *P < 0.05; **P < 0.01 by t test for comparison of TNF or PEP005 treated EC to untreated cells. c Release of IL-8 into culture supernatant assessed by luminex assay. Both PEP005 and TNF-α increased IL-8 release from EC compared to untreated EC. Data are mean ± SEM of three experiments. **P < 0.01 by t test for comparison between TNF-α or PEP005 treated cells with untreated cells