Fig. 1.
CAR transduction efficencies and natural 2B4 expression. a The various CAR constructs are depicted schematically. Following retroviral transduction of non-specifically activated peripheral blood mononuclear cells with 14.G2a-specific T cells, gene expression was determined by flow cytometric quantification of F(ab′)2-positive cells. To quantify the transduction efficiencies of CD3+ T cells (upper panel), the gate included all vital lymphocytes. For CD4+ and CD8+ T cells, gates were set on CD3+/CD4+ or CD3+/CD8+ cells, respectively. b Expression of 2B4 on CD4+ and CD8+ T cells measured by flow cytometry on day 14 after non-specific stimulation. Shown is one representative experiment of three