Fig. 2.

Therapeutic effects of AC and HER-2/neu DNA vaccination in MBT-2 tumor-carrying C3H/HeN mice. a The treatment regimen for mice treated with the HER-2/neu DNA vaccine–AC combination. Ten days after s.c. MBT-2 cell inoculation, AC was administered orally daily and the mice were vaccinated with hN’-neu DNA vaccine three times at weekly intervals using a gene gun. b The tumor volume was measured at the indicated time. Mean values of five to eight mice per group ± SEM are shown. c The Kaplan–Meier survival curve for the different groups of tumor-challenged mice. Asterisk indicates a statistically significant difference when compared with control vector group (p < 0.05). Double asterisks indicate a statistically significant difference when compared with the hN’-neu DNA vaccine alone group (p < 0.05). The experiments were repeated two times and similar results were obtained