Table 1.

Identification of bovine proTα fragments generated by trypsin¹. Fragments T1–T5 were isolated by RP-HPLC (Rt, retention time) and their molecular weight (MW) and primary structure (sequence) were determined by mass spectrometry

Fraction number	Rt [min]	MW [in Da]	ProTα residues	Sequence
T1	5.7	849.24	103–109	QKTDEDD
T2	15.0	1564.58	89–102	AAEDDEDDDVDTKK
T3	18.1	1130.44	21–30	EVVEEAENGR
T4	22.9	1466.74	1–14	SDAAVDTSSEITTK
T5	26.9	6144.07	31–87	EAPANGNANEENGEQEADNEVDEEEEEGGEEEEEEEEGDGEEEDGDEDEEAEAATGK

¹ Bovine proTα: acSDAAVDTSSEITTK ↓ DLKEKK ↓ EVVEEAENGR ↓ EAPANGNANEENGEQEADNEVDEEEE

Human proTα: acSDAAVDTSSEITTK DLKEKK EVVEEAENGR DAPANGNANEENGEQEADNEVDEEEE

EGGEEEEEEEEGDGEEEDGDEDEEAEAATGK ↓ R ↓ AAEDDEDDDVDTKK↓QKTDEDD

EGGEEEEEEEEGDGEEEDGDEDEEAE S ATGK R AAEDDEDDDVDTKK QKTDEDD

Primary structures of bovine and human proTα are overlayed. Arrows (↓) indicate where tryptic cleavages occurred

Bold letters indicate differences in the amino acid sequences of the two polypeptides