Fig. 2.

Infiltration and activation of NK cells after intratumoral injection of HVJ-E. a Intratumoral infiltration of immune cells was investigated by quantitative real-time RT-PCR (n = 5). DX5 mRNA expression showed a marked increase. SE < 5%. This experiment was repeated three times with similar results. b NK cell infiltration. Immunofluorescence staining using a FITC-conjugated anti-mouse CD49b (DX5)/Pan NK cell monoclonal antibody shows prominent infiltration of DX5-positive cells (green) into an HVJ-E-treated tumor (×200). This experiment was repeated three times with similar results. c Activation of intratumoral NK cells: NK cells were purified from tumors injected with HVJ-E or saline, and IFN-γ and CD69 mRNA expression was assayed by quantitative real-time RT-PCR. Both IFN-γ and CD69 expressions were upregulated in HVJ-E-treated tumors. SE < 5%. This experiment was repeated three times with similar results. d NK cytotoxity in vivo. Cytotoxicity assays were performed with NK cells harvested from the spleens of mice after treatment with HVJ-E (filled square) or saline (filled diamond). NK cells from HVJ-E-treated mice showed an increase of cytotoxity against Renca cells. This experiment was repeated three times with similar results