Fig. 4.
TNF-α and IFN-γ release by Vγ9Vδ2 T cells. PBMCs from two patients (1 CRC and 1 HCC) and two donors were treated at day 0 with 300 nM BrHPP and cultured in presence of 400 IU IL-2/ml within 2 weeks. About 3 × 105 expanded Vγ9Vδ2 T cells were co-cultured with 105 cells from CRC (C181, C187, and SW620), HCC (HepG2, HuH7) and Daudi cell line in 2 ml of complete medium. TNF-α and IFN-γ release was measured, respectively, 6 and 48 h after stimulation in culture supernatants by ELISA. Control was medium alone. Representative data obtained with patient C322 are shown