Table 2.
Large-scale expansions in closed bag systems of γδ T cells from donor PBMCs with BrHPP
| Day 0–4 | Day 15–17 | ||||
|---|---|---|---|---|---|
| PBMC seeded (×109) | γδ T cells (%) | Cells recovered (×109) | γδ T cells (%) | γδ T cell expansion index | |
| Cyta1 | 1.2 | 2 | 16 | 82 | 547 |
| Buf1 | 1 | 1 | 9.6 | 81 | 778 |
| Buf2 | 0.491 | 5 | 14 | 88 | 502 |
| Buf3 | 0.421 | 2 | 16.5 | 91 | 1,783 |
| Vpunc1 | 0.136 | 7 | 5.6 | 98 | 576 |
| Mean ± SD | 88 ± 6 | 837 ± 539 | |||
PBMC were isolated from cytapheresis (Cyta), buffy-coat (Buf) or 100 ml venous peripheral blood punction (Vpunc). The percentage of cells expressing the γδ-TCR was determined by flow cytometric analyses using anti-pan-γδ mAb, at the beginning (day 0–4) and at the end (day 15–17) of the culture. γδ T cell expansion index = number of γδ T cells recovered/number of γδ T cells seeded