Fig. 1.

Flow cytometric analysis of circulating DCs and myeloid and plasmacytoid DCs in freshly isolated PBMC obtained from one healthy donor (A) and one cancer patient (B). PBMC were stained with a lineage cocktail of FITC-conjugated MoAb (CD3, 14, 16, 19, 20, and 56) and a PC5-conjugated anti-HLA-DR MoAb. DCs were detected as lineage negative and HLA-DR as positive (Lin-DR+) cells. For further analysis of DC subsets, myeloid and plasmacytoid DCs were identified by the expression of PE-conjugated CD11c and CD123 among Lin-DR+ cells, respectively. The total number of PBMC acquired for analysis was 154,093 cells in (A) and 127,579 cells in (B)