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Real-time RT-PCR of total ascites cells and of the cell line established from ascites. cDNA was analyzed for expression of melanoma tumor antigens gp100, MAGE-3, Melan-A, and NA17-A, and for β-actin by real-time RT-PCR. a Real-time RT-PCR from total ascites cells. b Real-time RT-PCR from the melanoma cell line
